Mix & Go! XJa (DE3) Autolysis Competent CellsWhile there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage R gene, encoding the lysozyme, also known as endolysin, is induced during growth. Cells are harvested

Use with Vortex-Genie® 1

and plants

5 ml dry volume and the option of 2 mm or 0

The marker is supplied in water and can be stained with dyes specific for single-stranded nucleic acid species e

It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press)

The non-methylated pUC19 DNA is pUC19 isolated from a methylation-negative strain of bacteria (Dam–

The set is ideal as a “spike-in” control to assess bisulfite conversion efficiency within the same reaction as the sample

Robust reverse transcription is achieved even with challenging templates that are GC-rich or contain secondary structures

sequencing etc

The RNA Clean & Concentrator-5 kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality

Zymo-Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR

Component used in the OneStep-96 PCR Inhibitor Removal kit (Cat #D6035)